New PILOT Publication in Science translational Medicine
PILOT members Florian Jaudas, Huan Liu, Christian Schulz have published a new article in Science Translational Medicine.
Editor’s summary
The use of CFTR modulators for the treatment of cystic fibrosis has led to substantial improvement in lung function, but problems with inflammation and infection persist. In Jaudas et al., the authors characterized the innate immune systems in CFTR-deficient pigs and found higher pulmonary infiltration of monocytes compared with that in wild-type pigs, even at birth. Transcriptional and functional analysis of peripheral blood mononuclear cells from neonatal pigs and preschool aged children with cystic fibrosis suggested that emergency myelopoiesis led to an increase in less mature monocytes with reduced phagocytic activity. These results indicate that additional, CFTR-independent therapeutic strategies may be needed to address these complications. —Allison Williams
Abstract
In patients with cystic fibrosis (CF), repeated cycles of infection and inflammation eventually lead to fatal lung damage. Although diminished mucus clearance can be restored by highly effective CFTR modulator therapy, inflammation and infection often persist. To elucidate the role of the innate immune system in CF etiology, we investigated a CF pig model and compared these results with those for preschool children with CF. In newborn CF pigs, we observed changes in lung immune cell composition before the onset of infection that were dominated by increased monocyte infiltration, whereas neutrophil numbers remained constant. Flow cytometric and transcriptomic profiling revealed that the infiltrating myeloid cells displayed a more immature status. Cells with comparably immature transcriptomic profiles were enriched in the blood of CF pigs at birth as well as in preschool children with CF. This pattern coincided with decreased CD16 expression in the myeloid cells of both pigs and humans, which translated into lower phagocytic activity and reduced production of reactive oxygen species in both species. These results were indicative of a congenital, translationally conserved, and functionally relevant aberration of the immune system in CF. In newborn wild-type pigs, CFTR transcription in immune cells, including lung-derived and circulating monocytes, isolated from the bone marrow, thymus, spleen, and blood was below the detection limits of highly sensitive assays, suggesting an indirect etiology of the observed effects. Our findings highlight the need for additional immunological treatments to target innate immune deficits in patients with CF.
Full article: https://doi.org/10.1126/scitranslmed.adk9145